Affiliation:
1. Fujian Provincial People's Hospital
2. The Affiliated People's Hospital of Fujian University of Traditional Chinese Medicine: People's Hospital of Fujian Province
3. Fujian University of Traditional Chinese Medicine
4. Case Western Reserve University
5. Fujian Medical University Union Hospital
6. Fujian Provincial People's Hospital: People's Hospital of Fujian Province
Abstract
Abstract
Purpose
Xijiao Dihuang decoction (XJDHT), a traditional Chinese medicine, is widely used to treat patients with sepsis. However, the mechanisms underlying the effects of XJDHT on cardiac dysfunctionhave yet to be fully elucidated. The present study evaluated the potential utility of XJDHT in protecting against sepsis-induced cardiac dysfunction and myocardial injury.
Methods
Mice administered Lipopolysaccharide (LPS,10 mg/kg) or equivalent saline solution (control) were treated with XJDHT (10 g/kg/day) or saline by gavage for 72 hours.
Results
Treatment with XJDHT significantly enhanced myocardial function and attenuated pathological change, infiltration of inflammatory cells, levels of TNF-α,IL-1β and expression of TLR4 and NF-κB in mice with sepsis. RNA sequencing and Kyoto Encyclopedia of Genes and Genomes pathway analyses identified 531 differentially expressed genes and multiple enriched signaling pathways including the PI3K/AKT pathway. Further, XJDHT attenuated cardiac apoptosis and decreased Bax protein expression while increasing protein levels of Bcl-2, PI3K, and p-AKT in cardiac tissues of mice with sepsis.
Conclusion
In summary, XJDHT improves cardiac function in a murine model of sepsis by attenuating cardiac inflammation and apoptosis via suppression the TLR4/NF-κB pathway and activating the PI3K/AKT pathway
Publisher
Research Square Platform LLC