Expression and biological functions of tRNA-Derived Fragments Secreted by Extracellular Vesicles in sepsis-associated ARDS

Abstract

Abstract Objective: To detect the expression level of tRNA-derived fragments secreted by extracellular vesiclesin sepsis and explore the influence of tRNA-derived fragments on the occurrence of sepsis. Methods: High-throughput sequencing was performed to examine the tRFs belonging to the H-EV(Extracellular Vesicles derived from the plasma of healthy human) and S-EV (Extracellular Vesicles derived from the plasma of septic patients)groups. The expression of seven differentially expressed tRNAs were detected by qRT-PCR. Finally,Target prediction and bioinformatics analysis were performed to explore their co-targeted genes and biological functions. Results: It was found that a variety of differentially expressed tRFs in the extracellular vesicles from sepsis patients by sequencing. There were 1 upregulated and 22 downregulated tRFs in S-EV group,respectively.Higher expression levels of plasma exosomal tiRNA-1:34-Glu-CTC-1-M2, and lower expression levels of tRF-52:71-chrM.Pro-TGG,tRF-1:28-chrM.Ser-TGA,tRF-60:76-Lys-TTT-3-M2,tRF-58:75-Cys-GCA-11-M7,tRF-1:15-Val-TAC-1-M3,tRF-59:76-Tyr-GTA-1-M2 in S-EV group were confirmed by qRT-PCR. Conclusion: The tiRNA-1:34-Glu-CTC-1-M2 was over-expressed in S-EV group,which might be a diagnostic biomarker for the diagnosis and monitoring of sepsis.