Abstract
Purpose To investigate the effects of chromosomal aneuploidy on preimplantation embryos’ development by analyzing the morphological and morphokinetic parameters between euploid embryos and aneuploid embryos.Methods Conducted a retrospective analysis of the morphological and morphokinetic parameters of embryos cultured in the Embryo Scope Plus time-lapse incubator and underwent trophectoderm cell sampling for preimplantation genetic testing (PGT) at the Reproductive Center of the First Affiliated Hospital of Hainan Medical University from June 2019 to September 2022. Statistical methods, including the Mann-Whitney U test and Chi-Square test, were used to analyze the data.Results 1. A total of 1888 embryos from 487 cycles were included, with 724 being euploid embryos (38.30%) and 1164 being aneuploid embryos (61.70%). 2.In embryos that has become blastocysts, aneuploidy of embryonic genome had no effect on morphology of D1 and D2 embryos, had moderate effect on D3 embryos but significantly affects blastocysts, the worse the Gardner score, the higher the aneuploidy rate. 3. The influence of aneuploidy on the morphokinetic of the embryo becomes apparent at t5, with euploid embryos exhibiting faster development compared to aneuploid embryos. 4.There is no evidence to suggest that single chromosome aneuploidies, such as chr-3, chr-7, chr-8, chr-10, chr-11, chr-12, chr-13, chr-15, chr-18, chr-20, and chr-21, has any influence on the morphokinetic of embryos.Conclusion Genomic aneuploidy has an impact on embryo development, but not every stage is affected. Additionally, certain specific single chromosome aneuploidies does not influence embryo development during the preimplantation stage.