Association between host defense peptide IDR-1002 and ciprofloxacin: effects on human dental pulp cells

Abstract

Abstract Aim: to evaluate the effects of the association of host defense peptide IDR-1002 and ciprofloxacin on human dental pulp cells (hDPSCs). Materials and methods: hDPSCs were stimulated in culture with ciprofloxacin and IDR-1002. Cell viability (by MTT assay), migration capacity (by scratch assay), production of inflammatory and anti-inflammatory mediators by hDPSCs (by RT-PCR) and osteogenic differentiation (after alizarin red staining) were evaluated. Statistical differences were verified by one-way ANOVA and Bonferroni post-tests. Results: phenotypic profile of hDPSCs demonstrated more than 97% for positive marked mesenchymal stem cell. Increased pulp cells migration and proliferation were observed after 24 and 48h of exposure to IDR-1002 with and without ciprofloxacin. Mineral matrix formation by hDPSCs was observed in the presence of the association while its reduction was observed in the presence of peptide. After 24h of cell incubation, the association between ciprofloxacin and IDR-1002 significantly downregulated TNFRSF-1, IL-1β, IL-8, IL-6, and IL-10 gene expression (p ≤0.0001). Conclusions: The association between the IDR-1002 and ciprofloxacin showed favorable immunomodulatory potential, emerging as a promising option for pulp revascularization processes. Clinical relevance: These results appear in addition to previous results, increasing the potential of this synergistic association for biotechnological applications in the context of pulp revascularization.