MiR-200c inhibits the expression of zinc finger E-box-binding homeobox 1 (ZEB1) to suppress bladder cancer cell migration and invasion

Abstract

Abstract MiR-200c functions to modulate cell growth and differentiation and alters the miR-200c expression related to cancer development and metastasis. This study evaluated the role of miR-200c in bladder cancer cells and the underlying molecular events. Methods:Quantitative RT-PCR was used to determine miR-200c expression in bladder cancer cell lines. Gene transfection was performed to stably restore miR-200c expression or silence miR-200c expression, and the cells were then subjected to cell viability, transwell migration and invasion, and luciferase assays. Results:The miR-200c level was significantly downregulated in bladder cancer cells, whereas the restoration of miR-200c expression suppressed tumor cell migration and invasion capacity. Antagonizing miR-200c drastically acceleratedtumor cellinvasion, migration capacity. At the gene level, miR-200c targeted zinc finger E-box binding homeobox 1( ZEB1) expression, which resulted in a concomitant increase in E-cadherin levels. Conclusion The data from the current study demonstrated that miR-200c targeted the expression of the cell migration and invasion regulator ZEB1 and that the over-expression of miR-200c suppressed bladder cancer cell migration and invasion ability, indicating that miR-200c possesses an anti-tumor progression function in bladder cancer.