Comparative cytotoxicity, immunomodulatory and anti-inflammatory potential of FMB (Fuzed, Micronized and Bio-activate) and conventional extracts of Glycyrrhiza glabra

Abstract

Abstract Essential trace elements (ETEs) are important for the human body as like plant phytoconstituents in the products or extracts perform cell functions at biological, chemical and molecular levels. ETEs serve as cofactors for numerous enzymes and as centres for maintaining the structural integrity of proteins and enzymes as well as mediating significant biological processes. Therefore, ETEs are required in our therapeutic regimen to attain maximum efficacy. In the present study, we have successfully replenished the ETEs into a conventional extract of Glycyrrhiza glabra by utilizing an advanced technology called FMB [(fusion (F), micronization (M), bioligation or bioactivate (B)] and investigate their cytotoxicity, immunomodulatory and anti-inflammatory activities. The elemental analysis confirmed that FMB technology significantly replenishes iron, zinc and calcium in the conventional extract. Results of cytotoxicity revealed that FMB extract is safer than conventional extract. Moreover, significant pinocytic activity was observed in FMB extract as compared to conventional extract while similar results were observed in a splenocyte proliferation assay. In the case of anti-inflammatory activity, FMB extract showed better activity in comparison to conventional extract. Conclusively, the current study finds that FMB approaches can be a more effective substitute for conventional extracts by offering them a higher therapeutic value.