Effect of sY1192 single-locus microdeletion in the AZFb/c region on spermatogenesis and pregnancy outcomes

Author:

Chen Gangxin1ORCID,Sun Yan2,Chen Yongyan2,Yang Rui2,Li Haiyan2,Zheng Beihong2

Affiliation:

1. Fujian Maternal and Child Health Hospital Department of Traditional Chinese Medicine: Fujian Provincial Maternity and Children's Hospital Department of Traditional Chinese Medicine

2. Fujian Maternity and Child Health Hospital College of Clinical Medicine for Obstetric & Gynecology and Pediatrics: Fujian Provincial Maternity and Children's Hospital

Abstract

Abstract Backgroud: Y chromosome microdeletions is an important cause of male infertility. At present, research on the Y chromosome mainly focuses on analyzing the loss of large segments of the AZFabc gene, and few people have studied the impact of unit point deletion in the AZF band on fertility. Methods: A total of 15 associated sequence tag site loci in the AZF region of the Y chromosome in 2,000 patients with oligoasthenospermia were tested, and 116 patients with AZF microdeletion were selected. Calculated the proportion of azoospermia caused by the sY1192 deletion. The proportion and type of sY1192 independent and combined deletions in the AZFb/c region were determined to analyze the changes in the associated sex hormone levels due to the loss of sY1192 and the pregnancy outcomes of patients with weak or obstructive assisted reproductive techniques to assist in pregnancy. Results: Among the 116 patients with AZF microdeletion, the sY1192-independent deletion accounted for 41.38% (48/116), and the sY1192 combined deletion with other loci accounted for 25.86% (30/116). Eight patterns were found in the deletions associated with sY1192: the independent deletion of sY1192 (61.5%, 48/78); the common deletion of sY1192 and sY153 (1.3%, 1/78); the common deletion of sY1192, sY153, sY254, and sY255 (26.9%, 21/78); the common deletion of sY1192, sY121, sY127, and sY134 (1.3%, 1/78); the common deletion of sY1192, sY121, sY127, sY134, sY153, sY254, and sY255 (1.3%, 1/78); the common deletion of sY1192, sY121, sY127, sY134, sY153, sY254, sY255, and sY160 (3.8%, 3/78); the common deletion of sY1192, sY105, sY121, sY134, sY152, sY153, sY254, sY255, and sY160 (1.3%, 1/78); and 15 missing loci (2.6%, 2/78). The sperm detection rate was similar in the semen of patients with sY1192 independent deletion and sY1192 combined deletion (52.1% vs. 50.0%, P > 0.05). The sperm detection rate was higher than that in other types of microdeletions present at the sY1192 locus (52.1% vs. 32.0%, P < 0.05). The hormone levels were similar in patients with sY1192 deletion alone and in those with sY1192 deletion and other types of microdeletions in the presence of the sY1192 locus (P > 0.05), but the Follicle stimulating hormone (FSH), Luteinizing hormone (LH), Estradiol (E2),andTestosterone (T) were higher than those in the normal human controls (P < 0.05). After multiple intracytoplasmic sperm injection (ICSI) techniques, the total pregnancy rate of sY1192 independent deletion was similar to that of other types of microdeletions (73.9% vs. 75.0%, P > 0.05), but the fertilization rate and cleavage rate were higher (P < 0.05). Conclusions: Eight deletion patterns were observed for sY1192 microdeletions of AZFb/c, dominated by the independent deletion of sY1192. Combined deletions mostly occurred in sY1192, sY153, sY254, and sY255 (the b2–b4 amplicon region distributed in the AZFc region). The FSH, T, E2, and LH levels in patients with sY1192 deletions were higher than those in normal subjects and were not different from other types of microdeletions. After ICSI, the fertilization rate and cleavage rate of the sY1192 independent microdeletion were higher than those of other Y chromosome microdeletion types, but there was no difference in pregnancy outcomes.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3