Genomic analysis of a new lytic Salmonella Typhimurium bacteriophage (vB_StyS-sam)

Abstract

Abstract Salmonella spp. antibiotics resistance increase is a global problem. Currently, S. Typhimurium is the world's second leading etiology of salmonellosis with resistance to ampicillin, chloramphenicol, and trimethoprim-sulfamethoxazole. Bacteriophages have the potential to controlling Salmonella infections. In this study, the genome of a virulens Salmonella phage (vB_StyS-sam) isolated from Toshan backwater in Gorgan city of Golestan province in Iran (N364737 E542603) was analyzed. It contained 43221 base pairs with 49.77 (G + C) content that coded 66 proteins with eight promotor sequences. Twenty-four ORFs encoded functional proteins (replication, modification, and regulation). Eight unique ORFs with no similarity with any protein in the NCBI GenBank were found in this phage. Moreover, 29 Rho-dependent terminators are located in vB_StyS-sam phage genome. An anti-CRISPER sequence was found in the genome and BLASTn indicated that the vB_StyS-sam has homology with other Salmonella phages such as vB_SenS-Ent1 and other members of Jerseyvirus phages. Phage genome did not contain any antibiotic resistance and virulence genes. Thus, we suggested vB_StyS-sam phage has the potential to be used in phage therapy.