Preliminary exploration of the effects of environmental factors on the microsatellite status of BRAF mutated colorectal cancer

Author:

Tian Binle1,Chen Guiming1,Shi Xiaoqin1,Jiang Liren1,Jiang Tao1,Li Qi1,Yuan Lin2,Qin Jian1

Affiliation:

1. Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine

2. Shanghai Jiao Tong University School of Medicine

Abstract

Abstract Background To investigate the expression of EBV products and incidence of Gallstone disease (GD) among different microsatellite status in colorectal cancer (CRC) with BRAFV600E mutation. Methods We collected 30 CRC patients with BRAFV600E mutation and 10 BRAF (-) CRC patients as well as 54 healthy subjects. Tumor tissue samples were collected to detect the mutation of BRAF, KRAS, and TP53. Microsatellite status was determined by immunohistochemistry and PCR. EBER in situ hybridization was performed to detect EBV. In addition, we also collected clinical information about the patients. Results We found that although EBV products were detected in CRC, there were no significant differences in the EBV distribution between the different BRAF groups. Our study demonstrated that BRAFV600E mutation and BRAFV600E with MSI were significantly more frequent in the right CRC. Furthermore, the KRAS mutation rate in the BRAF-wild type group was proved to be significantly higher than that in the BRAF mutation group. In addition, we revealed that BRAF mutation and MSI were independent risk factors of TNM stage. The incidence of GD was higher in CRC patients than in general population, and although there was no significant difference between CRC with or without BRAFV600E mutation, the highest incidence of GD was found in MSS CRC with BRAFV600E mutation. Conclusions EBV plays a role in CRC, but is not a determinant of different microsatellite status in CRC with BRAFV600E mutation. The incidence of GD in MSS CRC with BRAFV600E mutation is significantly higher than that in the general population.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3