Simultaneous Production of Vitamin K2 and Iturin A by Bacillus velezensis ND

Author:

Shen Yeqiao1,Cao Xiaojie1,Tang Zhongmin1,Gao Yuqiu1,Zhang Huili1

Affiliation:

1. Shihezi University

Abstract

Abstract Bacillus velezensis is known to produce a diverse array of secondary metabolites, including the ability to synthesize lipopeptides such as iturin A, and additionally produce higher amounts of vitamin K2. Consequently, we sought to employ co-production fermentation to simultaneously generate vitamin K2 and iturin A. In order to significantly reduce fermentation costs and enhance substrate utilization efficiency, this article initially established a straightforward and effective product extraction method that enables the separate collection of vitamin K2 and iturin A from the fermentation broth. Simultaneously, the impacts of glycerol, soybean meal powder, yeast extract powder, and L-glutamic acid sodium, employed as carbon and nitrogen sources in the culture medium, on the co-production of vitamin K2 and iturin A were refined by optimizing factors such as culture temperature, initial pH and the concentrations of trace elements Na+ and K+, as well as additional additives L-glutamic acid sodium. It was discerned that glycerol, soybean meal powder, yeast extract powder, and L-glutamic acid sodium significantly influenced the concurrent production of vitamin K2 and iturin A. As a result, a novel process for the efficient co-production fermentation of vitamin K2 and iturin A was established, thereby enhancing the value-added aspect of the fermentation process. Furthermore, the implementation of a two-compartment biofilm reactor synergistically elevated the co-production efficiency. The yields of vitamin K2 and iturin A reached 46.88 mg/L and 5.58 g/L, respectively. This study provides a theoretical framework and practical implications for the integrated double-product fermentation process.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3