Evaluation of the protective effect of cannabidiol in lead toxicity in mice

Abstract

Abstract Purpose Considering the significant toxicity caused lead (Pb), identifying substances that can prevent or alleviate the toxic effects of Pb is important. This study aims to evaluate the protective effects of an anti-inflammatory, antioxidant substance; cannabidiol (CBD) against Pb toxicity. Methods A total of 4 groups, each consisting 10 mice were used. Groups I and III were not exposed to Pb, while Pb exposure was induced in groups II and IV by adding 1 gr/L of Pb to the drinking water. CBD was administered daily via intragastric gavage at a 25 mg/kg dose in groups III and IV. At the end of 30 days, the mice were euthanized, and blood and liver, kidney, testis tissue samples were collected. Levels of oxidative stress markers GSH, CAT, MPO, and pro-inflammatory cytokines TNF-α, IL-1β, IL-6 were measured using ELISA kits. Histological evaluation of the tissues samples was performed. Results Comparison between groups II and IV showed that CBD alleviated the oxidant and inflammatory damage caused by Pb as blood CAT, GSH levels increased and MPO, TNF- α, IL-1β, IL-6 levels decreased in CBD administered, Pb intoxicated mice compared to only Pb intoxicated mice. CBD also decreased the toxic effects of Pb in liver, kidney and testis tissues (p < 0.0001 for most comparisons). Comparison between groups III and IV revealed similar results as it showed that Pb reduced the beneficial effects of CBD. Conclusion This study showed that CBD has a protective effect against Pb toxicity. This finding indicates that CBD could be used as a food additive or supportive treatment for alleviating the toxic effects of Pb at risked population groups.