Multifunctional hydrogel containing platelet camouflaged ultra-small silver nanoparticles accelerates diabetic wound healing in Type 1 Diabetic rats

Abstract

Abstract Bacterial infection and impaired angiogenes are always great obstacles in the wound healing of diabetes. A diabetic wound causes thousands of infections or deaths around the world each year, so the problem of multidrug-resistant (MDR) cannot be ignored. Here, we synthesized a new ultra-small silver nanoparticles (usAgNPs) and conjugated usAgNPs with platelets (usAgNPs-PL) and loaded into CMC hydrogel (usAgNPs-PL/CMC hydrogel), and treated diabetic wound healing in streptozotocin-induced diabetic rat models. The usAgNPs-PL/CMC hydrogel was characterized by SEM, TEM and XPS spectra. The antibacterial ability was detected by XTT dyeing, ROS, bacterial biofilm assay, bacterial activity, et al. The results showed that usAgNPs had smaller particle size (13 nm) than traditional silver nanoparticles and evenly distributed. The Ag + released from usAgNPs can kill bacteria by interfering with bacterial intracellular metabolism; generation of ROS; damaging cell membrane integrity and inhibiting biofilm formation. And combined platelets were enhancing the angiogenesis of endothelial cells by regulating VEGF, PI3K-AKT. Together, this work describes the first example of constructing an all-in-one CMC hydrogel for antimicrobial diabetes wound treatment and showcases the promise of combined antimicrobials and angiogenesis achieved by using the CMC hydrogel in biomedicine applications.