Abstract
Numerous lifestyle factors contribute to the incidence of infertility, which is a major concern for couples throughout the globe. The effectiveness of in vitro fertilization (IVF) is highly dependent on the characteristics of culture media used. The present research aimed to determine the effects of L-aspartic acid, L-ascorbic acid, and silver nanoparticles (AgNPs) on the success rate of IVF in NMRI mice. The solutions of L-aspartic acid, L-ascorbic acid, and synthesized silver nanoparticles (AgNPs) were prepared. The 6–8 weeks-old NMRI male mice were used as sperm donors, and 6–8 weeks-old NMRI mice were used for oocyte donation. In addition, the IVF procedure was performed in 50 µL drops of KSOM + 15%BSA under mineral oil. Seven different experimental groups were investigated in addition to the control group. Oocytes were exposed to sperm for 4–6 h in the KSOM medium as a control group. According to the results, the groups containing supplements "AgNPs", "L-aspartic acid", and "AgNPs + L-ascorbic acid" were not suitable for the 2PN stage. In contrast, the group containing "L-aspartic acid + L-ascorbic acid" was the best group for the 2PN stage. In addition, supplementation of the mixture of L-aspartic acid and AgNPs solutions in KSOM + BSA culture medium can be considered as the most suitable group for the 2Cell stage. Gaining knowledge on how particular additives affect the success rates of IVF may lead to the development of advanced compositions, which will enhance assisted reproductive technologies (ART) throughout time and improve the lives of infertile couples.