TASP1 promotes the proliferation and metastasis of breast cancer by up-regulating ESR1

Abstract

Abstract Background Among females, breast cancer is the leading cause of cancer-related mortality. While the association between TASP1 and the development of various cancers has been reported, uncertainty remains regarding its role in breast cancer. Objective The goal of this study was to investigate the impact of TASP1 in breast cancer and its potential mechanism of action. Methods The expression of TASP1 in breast cancer tissues and cell lines was conducted through the utilization of RT-qPCR and western blot. Small interfering RNA (si-RNA) was used to silence the expression of TASP1 in breast cancer cell lines. The assessment of cell proliferation and metastasis ability was conducted by CCK-8 assay, colony-formation assay, wound-healing assay and transwell assay. Differential expression genes (DEGs) of TASP1 were downloaded using bioinformatics analysis, KEGG pathway enrichment analysis was performed to ascertain the connection of TASP1 and steroid hormone biosynthesis. Results The level of TASP1 expression was significantly elevated in both breast cancer tissues and cell lines. Silence of TASP1 in breast cancer cell lines resulted in the inhibition of cell proliferation and metastasis. KEGG pathway enrichment analysis revealed significant enrichment of steroid hormone biosynthesis. The silence of TASP1 led to a decrease in the expression of ESR1 and PGR, indicating their potential involvement in the regulation of TASP1. Conclusions TASP1 demonstrated upregulation in both breast cancer tissues and cell lines. The silence of TASP1 effectively impeded the proliferation and metastasis of breast cancer cells by suppressing ESR1.