Correlation of Interleukin-1 Receptor Antagonist (IL-1RA) gene polymorphisms in various cancers

Abstract

Abstract Background Cancer is the widely distributed deadly disease to plants and animals including humans. There are many genes or proteins involved cancer development specifically the oncogenes and tumor suppressor genes or mutant proteins. The interleukin-1 receptor antagonist cytokine genes are to modulate the interleukin-1 genes immune and inflammatory responses. The interleukin gene families also involved in the progression of tumorigenesis and angiogenesis of certain cancers development. Methods In this study, there are three kinds of cancers such as breast cancer, cervical cancer and lung cancers blood DNA isolated by salting out method and molecular identification of interleukin-1 receptor antagonist gene polymorphisms by using polymerase chain reaction and analysis in the agarose gel electrophoresis and finally the statistical analysis also performed to find the significance. Results The blood was collected from different cancer patients and done the DNA quantification and get the different sizes of DNA bands in 2% agarose gel. Further done the polymerase chain reaction method with of Variable Number Tandem Repeats (VNTR) of IL-1RA gene, from the result different sizes of DNA bands identified for confirmation of the IL-1RA genes responsible for against the cancer will increased significantly due to decrease in among all cancer types. The p-values of breast cancer, cervical cancer and lung cancer were p = 0.329, 0.190 and 0.346 respectively. The statistical analysis of genotype frequency of cervical cancer showed the greater significance. Conclusions In conclusion this is the novel and first report on IL-1RA in various cancers among south india. IL-1RA variable tandem repeat polymorphic profile might have served as the biomarker for the diagnosis purpose of the breast cancer and lung and may have the function in the host immune responses in the local and general environments of gynaecological cancers. IL-1RA decreases tumor growth and tumor angiogenesis.