LncRNA HOXD-AS1/ miR-182-5p / FOXO1 axis regulates invasion and migration of neuroblastoma cells

Abstract

Abstract The transcription factor forkhead box protein O1 (FOXO1) is critical for regulating cytokine and chemokine secretion. However, its function in the tumor microenvironment (TME) remains largely unexplored. In this study, we used Western blotting and qRT-PCR to detect the expression of FOXO1 in tissue samples and found that the FOXO1 gene was highly expressed in neuroblastoma cells. After knocking out FOXO1 in the neuroblastoma cell line, the abilities of invasion and migration were significantly weakened. The results of animal model experiments showed that, the volume of tumor tissue and the proportion of tumor cells in the knockout FOXO1 group were reduced, proving that the high expression of FOXO1 might contributes to neuroblastoma. To further study the regulatory network of FOXO1, according to TargetScran and related research, miRNA182-5p was screened to be able to target and bind to FOXO1. The expression of FOXO1 in the cell line overexpressing miRNA182-5p was significantly reduced, and the invasion and migration abilities of the cells were weakened. The database prediction results show that lnc RNA HOXD-AS1 can bind miRNA 182-5p. To verify whether there is a regulatory network for the lncRNA HOXD-AS1/miR-182-5p/FOXO1 axis, we constructed a lncRNA HOXD-AS1 knockout cell model and performed qRT-PCR and WB. The results showed that when the LncRNA HOXD-AS1 was knocked out, the expression of FOXO1 was inhibited, and the cell function showed a phenomenon of weakened invasion and migration, like the knockout of FOXO1. The results proved that the LncRNA HOXD-AS1/miR-182-5p/FOXO1 axis regulates the development of neuroblastoma cells.