Hsa_circ_0079474 facilitates epithelial-mesenchymal transition in intrauterine adhesion via miR-630/YAP1 axis

Abstract

Abstract Insufficient understanding exists of the molecular mechanisms underlying circRNA involvement in IUA and requires further investigation. This research aims to examine the role of hsa_circ_0079474 (circDGKB-009) and its potential mechanisms in intrauterine adhesion (IUA). A circRNA microarray was utilized to identify differences in circRNA expression between fibrotic endometrial samples and normal endometrial samples. Subsequent studies confirmed the expression and biological functions of hsa_circ_0079474 both in vivo and in vitro using various experimental techniques such as CCK-8, EdU, flow cytometry, FISH, RT-PCR, Western blot and IHC/ICC. The interactions between hsa_circ_0079474 and miR-630, as well as miR-630 and YAP1 were determined using dual-luciferase reporter assay and RNA immunoprecipitation. Hsa_circ_0079474 was dramatically elevated in IUA tissues compared to normal tissues. Hsa_circ_0079474 was found to enhance cell proliferation, expedite cell cycle progression, and facilitate epithelial-mesenchymal transition (EMT). Mechanistically, hsa_circ_0079474 acted as a sponge for miR-630, resulting in upregulation of YAP1 expression. This, in turn, promoted the progression of IUA. Hsa_circ_0079474 improves IUA by regulating the miR-630/YAP1 axis, providing a novel understanding of the molecular mechanisms underlying circRNA in IUA.