Bioinformatics analysis of programmed cell death in spinal cord injury

Author:

He Xuegang1,Deng Bo1,Ma Miao1,Wang Kerao1,Wang Yonggang1,Kang Xuewen1

Affiliation:

1. lanzhou university second hospital

Abstract

AbstractSTUDY DESIGN:Explanatory or mechanistic studiesOBJECTIVES:Programmed cell death (PCD) in the development of spinal cord injury (SCI) is complicated, including apoptosis, necroptosis, pyroptosis, ferroptosis, cuproptosis and autophagy. It is necessary to make clear the expression levels of PCD and potential molecular targets after SCI for formulating relevant treatment strategies.SETTING:Key Laboratory of Orthopedics Disease of Gansu Province, Lanzhou, Gansu,ChinaMETHODS:Download the rats SCI expression matrix GSE45006, ssGSEA method was used to analyze the PCD in SCI. Then the related differentially expressed genes (DEGs) were identified, and the gene ontology (GO) and pathway analysis, protein-protein interaction (PPI) network construction and hub genes were identified. Finally, the correlation between hub genes and PCD was analyzed.RESULTS:Apoptosis, necroptosis, pyroptosis, ferroptosis and autophagy increased significantly in the acute SCI, and then decreased gradually in the subacute and chronic stages; cuproptosis in acute SCI decreased significantly, and then gradually increased. In addition, we also screened 116 DEGs during the development of SCI. GO and pathway analysis showed that DEGs was related to mitosis and cell cycle. The identified hub genes are closely related to cell apoptosis, necroptosis, pyroptosis, ferroptosis after injury and autophagyCONCLUSIONS:PCD occurs differently in different stages after SCI. To inhibit apoptosis, necroptosis, pyroptosis, ferroptosis after injury and induce autophagy may be the therapeutic strategy. In addition, intervention therapy based on related hub genes may be the therapeutic target of SCI.

Publisher

Research Square Platform LLC

Reference67 articles.

1. Traumatic spinal cord injury: current concepts and treatment update;Rouanet C;Arq Neuropsiquiatr.,2017

2. Traumatic and Nontraumatic Spinal Cord Injuries;Ge L;World Neurosurg.,2018

3. Traumatic spinal cord injury;Ahuja CS;Nat Rev Dis Primers.,2017

4. Traumatic Spinal Cord Injury: An Overview of Pathophysiology, Models and Acute Injury Mechanisms;Alizadeh A;Front Neurol.,2019

5. Recent update on basic mechanisms of spinal cord injury;Quadri SA;Neurosurg Rev.,2020

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