Complete genome sequence of a novel mitovirus isolated from the fungus Fusarium oxysporum f. sp. ginseng causing ginseng root rot

Abstract

Abstract A novel Mitoviridaevirus, “Fusarium oxysporum mitovirus 2” (FoMV2), was isolated from the pathogenic Fusarium oxysporum f. sp. ginseng strain 0414 infesting Panax ginseng. The FoMV2 genome was 2388 nt in length and 30.57% in GC content. It carried an ORF encoding a 713-amino-acid RNA-dependent RNA polymerase (RdRp) with a molecular weight of 83.05 kDa. The homology between FoMV2 and the viruses isolated from “Botrytis cinerea mitovirus 8” and “Fusarium verticillioides mitovirus 1” was 87.94% and 77.85%, respectively. Phylogenetic analysis showed that FoMV2 belonged to Mitoviridae. To our knowledge, this is the first mycovirus isolated from F. oxysporum by ginseng root rot.