Antipsoriatic evaluation of a novel gel containing Chrysin nanostructured lipid carriers and probiotic in imiquimod induced mice model

Abstract

Abstract Psoriasis is a multifactorial skin disorder. Hence, its treatment using combination of Chrysin (CS) and probiotic (PB) could be effective strategy. In the present study, CS nano structured lipid carriers (NLCs) were prepared by modified hot-homogenisation method followed by sonication. Furthermore, it was converted into the gel after the addition of PB (Bifidobacterium infantis 35624) (BI) in it. The ex-vivo study was carried by Franz diffusion cell. The anti-psoriatic effect of the formulation was evaluated on imiquimod (IMQ) induced psoriatic mice. In addition, biochemical and histopathological analysis was done. The mice were divided into 11 groups (GP(n = 6)). All the groups except GP-1, received IMQ topically for 7 days and then treated as per study design. The groups were - GP-1 (Control), GP-2 (Experimental control), GP-3(Betamethasone cream), GP-4 (Blank NLCs gel), GP-5 (CS gel), GP-6 (CS NLCs gel), GP-7 (PB gel), GP-8 (CS L- PB loaded gel), GP-9 (CS H-PB loaded gel), GP-10(Optimized CS L NLC-PB loaded gel) and GP-11(Optimized CS H NLCs-PB loaded gel). The CS NLCs-PB loaded gel, CS gel alone has shown 30 µg/cm2/h, 50 µg/cm2/h permeability of CS within 24 h and 8h respectively. In the treated groups, GP 11 showed significantly (P < 0.001) higher efficacy by the reduction of psoriatic lesions, TNF-α, splenomegaly and Malonaldehyde when co-related with GP3, GP9, GP8, GP6, GP7 and GP5.The histopathological images of GP 11 revealed about the severe reduction of acanthosis and hyperkeratosis. The successful outcomes of study indicated that CS-NLCs-PB loaded gel was very effective in treating psoriasis in mice.