Affiliation:
1. Changsha Center for Disease Prevention and Control, Changsha, Hunan 410007
2. Public Health College, University of South China, Hengyang, Hunan 421001
3. Changsha Kaifu Disease Prevention and Control Center, Changsha, Hunan 410007
Abstract
Abstract
Background
We investigated the presence of Chlamydia psittaci in poultry and the environment in live poultry wholesale markets in Changsha during 2021–2022 and conducted phylogenetic analysis to understand its distribution in this market.
Methods
In total, 483 samples were analyzed using real-time polymerase chain reaction and 17 C. psittaci-positive samples using high-throughput sequencing, BLAST similarity, and phylogenetic analysis.
Results
Twenty two out of 483 poultry and environmental samples were positive for C. psittaci (overall positivity rate: 4.55%) with no difference in positivity rates over 12 months. Chlamydia psittaci was detected at 11 sampling points (overall positivity rate: 27.5%), including chicken, duck, and pigeon/chicken/duck/goose shops, with pigeon shops having the highest positivity rate (46.67%). The highest positivity rates were found in sewage (12.5%), poultry fecal (7.43%), cage swab (6.59%), avian pharyngeal/anorectal swab (3.33%), and air (2.29%) samples. The ompA sequences were identified in two strains of C. psittaci, which were determined to bear genotype B using phylogenetic analysis. Thus, C. psittaci genotype B was detected in the poultry and environmental samples from the poultry wholesale market in Changsha during monitoring.
Conclusions
Monitoring programs for C. psittaci in live markets need to be strengthened to address the possible zoonotic threat.
Publisher
Research Square Platform LLC
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