Proteomic identification of exosomes derived from psoriasis cells using DIA

Abstract

Abstract Psoriasis has become a common chronic disease, and protein molecules carried by exosomes play an important regulatory role in the disease. So far, there have been no reports on proteomic studies of exosomes derived from human psoriasis cells. Normal cultured keratinocyte line HaCaT was used as the control group, with a concentration of 10ng/mL of TNF α stimulating HaCaT to form psoriasis cells as the test group; Extract and prepare exosomes from the culture supernatant using magnetic bead method, and identify the exosomes using TEM, NTA, and WB; The data-independent acquisition (DIA) method was used to detect the proteomic profile of extracellular vesicles, and GO, KEGG, Rectom and PPI analyses were performed. A total of 2796 exosomal protein molecules were identified. Compared with the control group, the test group had 131 significantly differentially expressed proteins (DEPs), of which 59 were upregulated (LogFC > 1) and 72 were downregulated (LogFC<-1); Among them, upregulated proteins ADO, Cbx1, and downregulated protein ARGLU1 were first discovered as regulatory molecules related to angiogenesis, stress stress, and inflammation in psoriasis exosomes. KEGG enrichment analysis showed that DEPs in the exosomes of psoriasis cells are involved in the Focal adhesion signaling pathway of inflammation and abnormal angiogenesis crosstalk. The results of this study provide new information on proteins in the exosomes of psoriasis cells and contribute to understanding the mechanisms of psoriasis occurrence and development. The expression of proteins in exosomes derived from psoriasis cells is still poorly understood. To our knowledge, our study detected for the first time the proteomic characteristics of psoriasis cells, and for the first time discovered that psoriasis exosomes carry protein molecules ADO, Cbx1 and ARGLU1. These results provide new strategies for further studying the biological functions of exosomes in the occurrence and development of psoriasis.