Association between TIMP1 gene polymorphism and glioma in a northern Chinese population

Abstract

Abstract Aims To explore the association between two polymorphisms of TIMP1 and the risk of glioma in the northern Chinese population. Methods Genotyping of TIMP1 polymorphisms (rs4898 and rs2070584) was performed in 310 glioma patients and 350 healthy controls (HCs) from north China using the SNaPshot sequencing technique. Survival data from patients with glioma were analyzed. Results The frequency of the rs4898-C, rs2070584-G alleles were significantly lower in male glioma patients than those in HCs (p < 0.05). The frequency of heterozygote genotype (CT) was significantly lower in the patient group than control (OR = 0.49, 95%CI = 0.30–0.80, P = 0.004). For rs2070584, the frequency of heterozygote genotype (GT) was significantly lower in the patient group than control (OR = 0.63, 95%CI = 0.42–0.95, P = 0.026). The frequency of hemizygote C on rs4898 was lower for WHO III~Ⅳ glioma cases than for HCs (OR = 0.36, 95%CI = 0.17–0.73, P = 0.004). Haplotype analysis showed TIMP1 C-G (rs4898-rs2070584) frequency was lower in male glioma patients (OR = 0.80, 95%CI = 0.66–0.98, P = 0.034). The risk of OS for glioma patients carrying the rs4898 TT genotype increased by about 1.377-fold in comparison with CC + CT genotype patients (95%CI = 1.044 ~ 1.817, P = 0.024). Similarly, the risk of PFS for glioma patients carrying the rs2070584 TT genotype increased by about 1.347-folds in comparison with GG + GT genotype patients (95%CI = 1.051 ~ 1.727, P = 0.019). Conclusions In conclusion, the present study suggested that genetic polymorphisms of TIMP1 were associated with glioma risk in the Chinese population.; Rs4898 CT heterozygote and hemizygous C might be protective against glioma; the haplotype C-G (Rs4898-rs2070584) provides nearly 80% protection towards the development of glioma. Furthermore, TIMP1 polymorphisms (rs4898 and rs2070584) might be an independent prognostic factor in glioma patients.