Simultaneous quantification of biogenic amines as biomarkers for Parkinson's disease by combining ultraviolet and integrated pulsed amperometric detectors

Abstract

Abstract We developed a method combining ultraviolet detection and integrated pulsed amperometric detection for Parkinson's disease diagnosis through the simultaneous quantification of dopamine, 5-hydroxyindolacetic acid, homovanillic acid, serotonin, 3,4-dihydroxyphenylacetic acid, norepinephrine, and epinephrine. All target components were completely separated within 40 min with 5% acetonitrile solution containing 8 mM HClO4 and 0.20 mM − 1-octanesulfonic acid, and showed limits of detection of 0.03–0.10 ng and limits of quantification of 0.10–0.30 ng with linear regression coefficients of 0.9998–1.0000. All inter-day and intra-day precision values were below 9.58%, and the average recoveries were 93.71–109.82% for mouse striatum samples. In a clinical sample application, the levels of the seven components in striatal brain tissue in a mouse model of Parkinson’s disease decreased significantly compared to those of a control group. It was also confirmed via orthogonal partial least squares discriminant analysis that the seven components are useful biomarkers. Our reversed phase–HPLC-UV-IPAD method is expected to be helpful as a simple and economic analytical method for biogenic amines and their metabolites as biomarkers of Parkinson’s disease in clinical and biological labs.