A model of pregnancy-associated malaria for inducing adverse pregnancy outcomes in ICR mouse

Author:

Zhang Yingying1,Liang Zhiming2,Xing Haoyu1,Yu Chuyi1,Liang Jianming1,Xu Qin1,Song Jianping1,He Zhouqing1

Affiliation:

1. Guangzhou University of Chinese Medicine

2. the Fifth Affiliated Hospital of Guangzhou Medical University

Abstract

Abstract Background Based on understanding of placental pathological features and safe medication in pregnancy-associated malaria (PAM), establishment of a stable pregnant mouse infection model with Plasmodium was urgently needed.Methods ICR mice with vaginal plugs detected were randomly divided into post-pregnancy infection (Malaria+) and uninfected pregnancy (Malaria) cohorts. Age-matched mice that had not been mated were infected as pre-pregnancy infection group (Virgin control), which were subsequently mated with ICR males. All mice were inoculated with 1×106 Plasmodium berghei ANKA-infected RBCs by intraperitoneal injection, and the same amount of saline was given to Malaria group. We recorded the incidence of adverse pregnancy outcomes and the amounts of offspring in each group.Results The Virgin group mice were unable to conceive normally, and vaginal bleeding, abortion, or stillbirth appeared in the Malaria+ group. The incidence of adverse pregnancy outcomes was extremely high and statistically significant compared with the control (Malaria) group (P < 0.05), of which placenta exhibited pathological features associated with human gestational malaria.Conclusions The intraperitoneal injection of 1×106 Plasmodium berghei ANKA-infected RBCs could establish a mouse model of post-pregnancy malaria infection.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3