Transcriptomic study of acute nitrite stress on ovary development stages of river shrimp (Macrobrachium nipponense)

Abstract

Abstract River shrimp (Macrobranchium nipponense) is a species of shrimp that is widely farmed in China. As the scale of farming expands, the demand for juvenile shrimp will also increase. Their ovarian development time is short, and they are easily affected by environmental factors. Nitrite is a common water pollutant. Excessive exogenous nitrite concentration can affect the gonad development of some animals. However, the effect of nitrite on ovarian development in crustaceans remains to be studied. This experiment conducted a transcriptomic study of ovaries in stage III of development by exposing M. nipponense (3.42 ± 0.12g) to nitrite in LC5048h (10.2 mg/L) for 48 hours. The results showed that the 11,592 differential genes (DEGs) included 3,243 up-regulated genes and 8,349 down-regulated genes. These DEGs are mainly enriched in pathways such as lipid metabolism, carbohydrate metabolism, lysosomal pathway and vitellogenesis. In order to resist nitrite stress, energy supply needs to be enhanced, and the expression levels of carbohydrate metabolism pathways and triglyceride synthesis and metabolism pathways increase. in the lysosomal pathway. The expression levels of Cathepsin L (CTSL) and Lysosomal aspartic protease were down-regulated. The expression levels of genes related to vitellogenin formation, juvenile hormone acid methyltransferase (JHAMT), vitellogenin receptor (VgR), and vitellogenin 2 (Vg2) were down-regulated. This shows that nitrite stress inhibits the ovarian development of M. nipponense, and there may be a mechanism similar to that of fish in regulating ovarian development by nitrite.