Dufulin activates OsDUF6 protein against salt stress in rice plant

Abstract

Abstract Background Dufulin is a chemical immune activator in rice plant. Soil salinity is one of the main environmental stresses in rice production. When plants are exposed to salt stress, a range of cellular equilibria will be disrupted. Previous studies have shown that Dufulin has a positive effect on salt tolerance in rice. Results In this study, we studied the mechanism of Dufulin in response to salt stress. Based on the transcriptome analysis of Dufulin in the process of salt tolerance in rice, we selected the OsDUF6 protein located on the cell membrane and studied its molecular function by overexpression of OsDUF6. The results showed that the salt-induced decreases in root, stem, and leaf length and increased leaf yellowing rate and Na+ concentration in the wild-type plant were improved in the overexpressed lines, and increased the enzyme activity of the SOD, POD, CAT and PAL. OsDUF6 played a positive role in Na + transport by comparing the growth of the salt-sensitive yeast mutant complemented with OsDUF6. In addition, RT-qPCR analysis confirmed that the overexpression of OsDUF6 significantly changed the expression level of genes related to growth and stress tolerance. Conclusions Combined with previously published data, our results supported that OsDUF6 is an important functional factor of Dufulin to promote salt stress resistance in rice and plays a role in promoting salt tolerance in rice.