Abstract
Here, we report the genome sequence of strain A2. The genome size was 3,855,926 bp, the GC content was 67.4%, and it contains 3509 genes, 62 tRNA, eight rRNA, and four snRNA. Phylogenetic analysis of the 16 S rRNA gene in the RDP, NCBI, and TYGS databases indicates that strain A2 belongs to Halomonas salifodinae. Also, MLSA analysis confirms that A2 is closely related to H. salifodinae. Phylogenomic and comparative genomic analysis using the ANIs and dDDH indicators classify H. salifodinae A2 and Bisbaumannia pacifica NBRC 102220 in a separate phylogenetic group of the genus Halomonas. The phylogenomic and pangenome analysis support the above, placing H. salifodinae A2 in a separate group with B. pacifica NBRC 102220. The pangenomic analysis shows 136,122 genes that comprise the pangenome with 317 core genes, 3457 shell genes, 132,332 accessory genome, and 691 unique genes. We found 29 genes for secretion systems in the genome analysis, 23 for Na + and K + ion transport, 6 BGC groups, a total of 12 genomic islands, an 8.2Kb gene prophage region, 15 regions associated with CRISPR and one CAS-TypeIF cas gene cluster region, 12 genes of biotechnological importance, 38 unique genes essential for adaptability and biotechnological relevance, as well as, 35 genes for the synthesis of compatible solutes. Furthermore, we propose the reclassification of the species within the genus Bisbaumannia.