Effect genotoxic, mutagenic and modulate the proteome from the exposition of alternative fuels.

Abstract

Abstract To determine the genotoxic, mutagenic effect and the ability to modulate the organic material extractable from diesel particulate matter mixed with alcohols (10% ethanol and butanol) (MOE), DNA damage was evaluated through the comet assay and mutagenicity. Using the AMES test on Salmonella typhimurium, proteomic analysis was carried out by extracting total proteins from cell cultures, integrity was assessed by one-dimensional (1D) electrophoresis, and protein profiles were obtained by two-dimensional (2D) electrophoresis. Image analysis in PDQuest reflected differentially expressed proteins which were characterized by mass spectrometry (MALDI-TOF/TOF), the protein-protein interaction network was identified with the STRING database, followed by complex detection analysis. through which it was possible to clarify the cell phones involved. The results showed that MOE caused DNA damage and mutagenicity in bacteria, and the Cox5A, TPI1, PRDX2, ERP29 and PDIA3 proteins were overexpressed and involved in processes related to oxidative stress and oxidative detoxification. In conclusion, exposure to these treatments modulates the proteome of the HepG2 cell line, evidencing detoxification processes at the cellular level.