Experimental study MMP10 Regulates the lymph node metastasis of Oral Squamous Cell Carcinoma Cells

Abstract

Abstract Aberrant MMP expression can be detected in almost all primary and recurrent tumors. The aim of this study was to identify the role of MMP10 in the cell biological function of OSCC. We analyzed the expression and survival data analysis were done using data from the Cancer Genome Atlas (TCGA) and the Clinical Proteomic Tumor Analysis Consortium(CPTAC) database, then expression of MMP10 in clinical OSCC samples was detected by using qRT-PCR. We reduced the expression of MMP10 to elucidate the effects of MMP10 on proliferation, migration and invasion. Genes associated with MMP10 were validated by qRT-PCR. The MMP10 expression was raised in HNSC and OSCC, and the high MMP10 expression in HNSC with a poor prognosis outcome. The MMP10 expression was upregulated in OSCC tissues when compared with adjacent tissues. Knockdown the expression of MMP10 inhibited the cell migration and invasion in vitro and lymph node metastasis in vivo. And the expression of MMP10 was regulated by WNT3A. The results of present study indicate that MMP10 plays a significant role in the invasion and migration of OSCC. MMP10 may be a possible target gene for the therapy of OSCC by inhibiting metastasis.