Abstract
In this study, Campylobacter jejuni phages were characterized and investigated the combined effects of phages and ethylenediaminetetraacetic acid (EDTA). The antibacterial activity of specific phages with/without EDTA was investigated at multiplicity of infection (MOIs) of 10 and 1000 at both 42 and 4 °C during 48 h of incubation. The phage cocktail with EDTA decreased the viable counts by 1 log and inhibited the regrowth of the phage-resistant population after a 48-h incubation at 42 °C. At 4 °C, a single phage, vB_CjeM-PC10, reduced the viable count of C. jejuni by 3 log and 2 log in Brain Heart Infusion (BHI) broth and on chicken skin, respectively, after incubation for 48 h. Genomic DNA sequence analyses showed that phage vB_CjeM-PC10 had a linear double stranded DNA (dsDNA) of 51,148 bp, and phage vB_CjeM-PC22 had a circular dsDNA comprising 32,543 bp. Since phages are temperate, they are not practically applicable without lysogenic gene disruption by genome editing. However, these findings enrich data resources by highlighting the potential control of C. jejuni infection.