Transcriptomics analysis of long non-coding RNAs in smooth muscle cells from patients with peripheral artery disease and diabetes mellitus

Author:

Yundung Yankey1,Mohammed Shafeeq2,Paneni Francesco2,Reutersberg Benedikt1,Rössler Fabian1,Zimmermann Alexander1,Pelisek Jaroslav1

Affiliation:

1. University Hospital of Zurich, University of Zurich

2. University Hospital Zurich and University of Zurich

Abstract

Abstract Diabetes mellitus (DM) is a significant risk factor for peripheral arterial disease (PAD), and PAD is an independent predictor of cardiovascular disorders (CVDs). Growing evidence suggests that long non-coding RNAs (lncRNAs) significantly contribute to disease development and underlying complications, particularly affecting smooth muscle cells (SMCs). So far, no study has focused on transcriptome analysis of lncRNAs in PAD patients with and without DM. Tissue samples were obtained from our Vascular Biobank. Due to the sample’s heterogeneity, expression analysis of lncRNAs in whole tissue detected only ACTA2-AS1 with 4.9-fold increase in PAD patients with DM. In contrast, transcriptomics of SMCs revealed 28 lncRNAs significantly differentially expressed between PAD with and without DM (FDR < 0.1). Sixteen lncRNAs were of unknown function, six were described in cancer, one connected with macrophages polarisation, and five were associated with CVDs, mainly with SMC function and phenotypic switch (NEAT1, XIST, MIR222HG, MIR100HG, HIF1A-AS3, MRI29B2CHG). The enrichment analysis revealed additional lncRNAs H19, CARMN, FTX, and MEG3 linked with DM. Our study revealed several lncRNAs in diabetic PAD patients associated with the physiological function of SMCs. These lncRNAs might serve as potential therapeutic targets to improve the function of SMCs within the diseased tissue and, thus, the clinical outcome.

Publisher

Research Square Platform LLC

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