Gooseberry Anthocyanins Alleviates Insulin Resistance by Regulating Ceramide Metabolism in High Fat Diet Mice

Abstract

Abstract In order to explore the effect and mechanism of Gooseberry anthocyanins (GA) on reducing the ceramide content to reduce Insulin Resistance (IR) in mice.Molecular docking, Real-Time PCR and Western Blotting were used to explore the molecular mechanism and the effects of different doses GA in high-fat diet (HFD) mice.The results showed that the binding energy of anthocyanins on CerS6 was in the range of -8.2 to 5.2 kcal/mol, with low energy parameters and good binding positions. GA could effectively inhibit visceral fat, liver index, TC, TG and LDL-C, and improved HDL-C, GSH-Px and SOD.GA decreased the level of Insulin sensitivity index from-5.15 to-4.54 and improved insulin sensitivity and insulin resistance in HFD mice. Real-Time PCR and Western blotting results showed that GA could reducemRNA levels of inflammatory factors IL-6 and TNF-α, inhibit the expression of CerS6,PKCζ, PPARγ, CD36 and enhance the expression of SphK2, Akt, p-Akt/Akt, ISR. This study revealed the molecular mechanism of improving insulin resistance of GA in S1P/Cer signaling pathway, and provided theoretical support for preventive treatment of obesity related diseases by polyphenols.