Bone turnover markers and transcriptomes changes induced by acute sleep deprivation

Abstract

Abstract [Absract] Objective: To explore the effect of acute sleep deprivation (SD) on the bone turnover status and the changes of gene expression in rat bone tissues. Methods: Wistar rats, 6 weeks age, were randomly divided into 3 groups: normal controls (NC) group, SD group, SD and recovery (SD+R) group. Acute SD model was established via a modified multi-level bench method. Bone turnover markers (P1NP, β-CTX) were measured. The femur tissues from NC and SD group were subjected to RNA sequencing. Differential expression genes were screened and the KEGG functional annotation analysis and GO enrichment analysis. Results: The serum PINP levels were significantly decreased after 72-hours SD intervention (p <0.05) and serum β-CTX levels were increased (p <0.05). There were 4441 differential genes (q-value value <0.05), of which 1985 genes were up-regulated and 2456 genes were down-regulated. Some genes related to osteogenesis and osteoclast differentiation such as Tnfrsf11a, Ctsk, spp1, and pth1r were significantly upregulated, and various signaling pathways, including bone resorption and bone remodeling signaling pathway were upregulated. KEGG enrichment analysis suggested that oxidative phosphorylation pathway and Alzheimer's disease pathway; while thyroid hormone signaling pathway, parathyroid hormone synthesis, secretion pathway, and insulin signaling pathway were significantly downregulated. Conclusions: This study found that acute SD rapidly breaks the balance of bone turnovers, and increased related mRNA expression. Sleep is essential for maintaining bone turnover status.