Affiliation:
1. Department of Pulmonary and Critical Care Medicine, Hainan Hospital of People’s Liberation Army (PLA) General Hospital
2. First Medical Center of Chinese PLA General Hospital
3. Chinese People’s Liberation Army (PLA) General Hospital
Abstract
Abstract
Objective: To compare microbiome in the lower respiratory tract of patients with bronchiectasis in North and South China.
Methods: Demographic and Sequencing data were collected from patients who underwent bronchoalveolar lavage fluid macro-genomic sequencing testing between September 2021 and September 2022 at Hainan Hospital of the General Hospital of the People's Liberation Army (Sanya, Hainan, South) and the Eighth Medical Center of the General Hospital of the People's Liberation Army (Haidian, Beijing, North). Sequencing data were analyzed, and patients were divided into subgroups according to their test results to compare microecological difference.
Results: We enrolled 75 patients, 42 in the North and 33 in the South. The number of smokers in the Northern group was higher than that in the Southern group and the FEV1/FVC(%) value was lower than that in the Southern group; otherwise, the groups had no significant differences. The most abundance bacteria in the lower respiratory tract of the Northern group were Pseudomonas aeruginosa, Klebsiella pneumoniae, and Nocardia wilsonii, while the Southern group had H. influenzae, P. aeruginosa, and Nocardia Gelsenkirchen. There were differences in the abundance of lower respiratory tract microbiome between patients with bronchiectasis in the Northern and Southern regions, depending on the severity of bronchiectasis.
Conclusion: The lower respiratory tract microbiome of our patients with stable bronchiectasis differed significantly between the North and South. The bacterial flora of the Southern group was closer to that reported in the international literature, while the core taxa of the Northern group was significantly different. P. aeruginosa and Klebsiella pneumoniae were most abundant in the Northern group, while H. influenzae and P. aeruginosa were most abundant in the Southern group, These findings can guide antibiotic treatment of exacerbations.
Publisher
Research Square Platform LLC