Author:
MEVADA Mevada S. D.,patel Patel H. U.,Mevada Shweta D.,mevada mevada shweta
Abstract
Abstract
A simple, rapid, accurate, economic and precise UV/VIS method has been developed and validated. Choices of a common solvent were essential so various solvent ranges including methanol, ethanol, Acetonitrile and phosphate buffer and various concentrations ranges of various buffers were analyzed. Hence methanol was selected as a solvent for the proposed method. Gallic Acid and Apigenin showed maximum absorbance at 217 and 268 nm respectively. Both drugs obey Beer Lambert’s law in the concentration range of 0.5-3 µg mL-1 for Gallic Acid and Apigenin respectively. The LOD and LOQ were found to be 0.00379 µg mL-1and 0.011µg mL-1for Gallic Acid, respectively. For, Apigenin the LOD and LOQ values were found to be 0.00801 µg mL-1 and 0.024µg mL-1 respectively. The method was quantitatively evaluated in terms of linearity, precision, precision, LOD, LOQ and recovery. The method is simple, convenient and suitable for the analysis of Gallic Acid and Apigenin in bulk drug.
Publisher
Research Square Platform LLC
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