Bifidobacterium animalis sup F1-7 and Lactobacillus plantarum FWDG alleviate the malnutrition of mice via ghrelin-GHSR/PKC pathway

Abstract

Abstract Purpose: Intestinal microecology was closely related to malnutrition, but the related mechanism was still unclear. This study aimed to reveal how microorganisms alleviated malnutrition via ghrelin-GHSR/PKC-SCFAs pathway. Methods and results: Melanoma cells B16F10-induced malnourished mice of lung cancer. Strains Bif. animalis F1-7 and L. plantarum FWDG alleviated the malnutrition of mice to some extent by increasing the dietary intake level of mice, but the effect of Bif. animalis F1-7 was more prominent. This process was through increasing the expression level of GHSR and PKC, reducing the expression level of IP3, and finally regulating the secretion level of ghrelin and improving the anorexia of mice. Meanwhile, Bif. animalis F1-7 also reduced the expression levels of GLUT2, SGLT1 and PEPT1 genes and proteins, promoted the increase of acetic acid, butyric acid, isovaleric acid and total acid levels, regulated the nutritional transport process of intestinal energy, and finally improved the nutritional status of tumor mice via ghrelin-GHSR/PKC-SCFAs pathway. Conclusion: Our study provided a data support for the application of potentially beneficial microorganisms of Bif.animalis F1-7 could acts as an auxiliary component to alleviate malnutrition.