Neuroprotective Effects of Tranexamic acid against hydrogen peroxide-induced Cytotoxicity on Human Neuroblastoma SH-SY5Y Cells

Author:

Niapour Ali1,Mohebi Yavar2,Ghalehnoei Hossein2

Affiliation:

1. Ardabil University of Medical Sciences

2. Mazandaran University of Medical Sciences

Abstract

Abstract Tranexamic acid (TA) is an anticoagulant drug that used worldwide. However, the adverse effects of TA may insult the nervous system. This study aimed to investigate detrimental and neuroprotective effects of TA on SH-SY5Y cells. In this study, SH-SY5Y cells were treated with various concentrations of TA and H2O2 for 24 hours. Neuroprotective effects of the TA were assessed on H2O2-challenged cells. SH-SY5Y cells were pretreated with TA for 12 hours and then exposed to H2O2 for 24 hours. Cell proliferation and cellular apoptosis was measured using the MTT Assay and Flow cytometry respectively. The results showed that TA at high concentrations diminished the cell viability and induced apoptosis via up-regulation of BAX and Caspase-3 genes expression and down-regulation of BCL-2 transcript. Also, phosphorylation of Akt was reduced after TA treatment. TA showed protective effects against H2O2-induced cell stress by down-regulating the expression of BAX and Caspase-3 genes, up-regulating the expression of BCL-2 and augmenting the p-AKT/AKT ratio. Our findings demonstrated that TA could exert its neuroprotective effects at lower concentration while at higher concentration induces apoptosis in SH-SY5Y cells.

Publisher

Research Square Platform LLC

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