Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds-Reference-Cited by-同舟云学术

Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds

Published:2008 Issue:2 Volume:23 Page:89-98
ISSN:1820-3949
Container-title:Pesticidi i fitomedicina
language:en
Short-container-title:PESTIC PHYTOMED

Author:

Balaz Jelica¹,Popovic Tatjana¹,Vasic Mirjana²^ORCID,Nikolic Zorica³

Affiliation:

1. Poljoprivredni fakultet, Novi Sad

2. Institut za ratarstvo i povrtarstvo, Novi Sad

3. Nacionalna laboratorija za ispitivanje semena, Novi Sad

Abstract

Xanthomonas axonopodis pv. phaseoli detection on artificially inoculated bean seeds was investigated. The method of the International Seed Federation - ISF (2006) was used. It included bacteria extraction from seeds and isolation on semiselective media with pathogenicity test of the investigated isolates. ELISA and PCR were used for verification of results. The results showed that the semiselective media MT (Milk Tween Agar) and XCP1 (Xanthomonas campestris pv. phaseoli Agar) were very suitable for isolation of X. a. pv. phaseoli. Pathogenicity was confirmed on young bean plants. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium X. a. pv. phaseoli.

Publisher

National Library of Serbia

Subject

General Medicine

Reference5 articles.

1. Detection of the Bean Common Blight Bacteria,Xanthomonas campestrispv.phaseoliandX. c. phaseolivar.fuscans,Using the Polymerase Chain Reaction

2. Milk–Tween agar, a semiselective medium for isolation and differentiation of Pseudomonas syringae pv. syringae, Pseudomonas syringae pv. phaseolicola and Xanthomonas axonopodis pv. phaseoli

3. Identificação de xanthomonas axonopodis pv. phaseoli e x. axonopodis pv. phaseoli var. fuscans através da técnica de pcr

4. Tween Media for Semiselective Isolation ofXanthomonas campestrispv.vesicatoriafrom Soil and Plant Material

5. Detecção de Xanthomonas axonopodis pv. phaseoli em sementes de feijão em meio de cultura semi seletivo

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