The ethanolic extract of Eryngium billardierei F. Delaroche restrains protein glycation in human serum albumin: an in vitro study

Abstract

Protein glycation is directly associated with many pathological conditions. This study investigated the potential of Eryngium billardierei extract to inhibit the glycation process in human serum albumin (HSA). After preparation of the ethanolic extract of E. billardierei, the structural changes of glycated HSA in the absence and presence of different concentrations of E. billardierei extract were investigated using circular dichroism (CD), fluorescence spectroscopy, and UV-Vis spectroscopy. The results confirmed that E. billardierei extract could reduce the formation of advanced glycation end products (AGEs) and Amadori products under in vitro glycation conditions and also improve HSA helical structure. In addition, a reduction in the HSA-cross amyloid formation was seen in the thioflavin T assay. The phytochemical analysis disclosed that E. billardieri extract is high in flavonoid and phenolic compounds. Accordingly, it could be concluded that the phenolics in E. billardieri extract could prevent glucose-induced HSA glycation. This study provides a rationale that E. billardieri extract could be implicated in controlling diabetes.