Affiliation:
1. Institut za kukuruz, Beograd
2. Institut za ratarstvo i povrtarstvo, Novi Sad
Abstract
Analyzing 167 non-processed seed samples of wheat, it was found that 145
samples (86.8 %) were contaminated with Tilletia species, while 22 (13.2 %)
samples were not contaminated. By using rep-PCR fingerprinting technique, it
was found that DNA isolates of T. tritici originated from Serbian wheat
samples had 80 % similarity with positive control for T. tritici. One isolate
shared similarity of 60% with T. tritici, T. controversa and T. laevis. It
was supposed that this isolate belongs to T. bromi. Isolate of T. laevis
shared a similarity of 70 % with isolates of T. tritici and T. controversa,
while T. walkeri was more than 10 % similar with T. tritici, T. controversa
and T. laevis. Although T. controversa and T. tritici had high percent of
genetic similarity, they were clustered separately. Our results suggest that
rep-PCR fingerprinting could be a useful tool for monitoring presence of
morphologically similar Tilletia species in wheat production areas.
Publisher
National Library of Serbia
Cited by
8 articles.
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