Affiliation:
1. State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, China
2. Dental Implant Center, West China College of Stomatology, Sichuan University, Chengdu, China
Abstract
To investigate whether adipose-derived stem cells (ADSCs) could be subject to neural differentiation induced only by Schwann cell (SC) factors, we co-cultured ADSCs and SCs in transwell culture dishes. Immunoassaying, Western blot analysis, and RT-PCR were performed (1, 3, 7, 14 d) and the co-cultured ADSCs showed gene and protein expression of S-100, Nestin, and GFAP. Further, qRT-PCR disclosed relative quantitative differences in the above three gene expressions. We think ADSCs can undergo induced neural differentiation by being co-cultured with SCs, and such differentia?tions begin 1 day after co-culture, become apparent after 7 days, and thereafter remain stable till the 14th day.
Publisher
National Library of Serbia
Subject
General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology
Cited by
8 articles.
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