Affiliation:
1. Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
2. Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
3. Department of Biotechnology, Urmia University, Urmia, Iran
Abstract
In the present study polymorphism of the exon 2 of MHC (Major
Histocompatibility Complex) gene in Makuie sheep breed was studied. Genomic
DNA from blood samples of 90 sheep was extracted and a 279 bp MHC exon 2
fragment was amplified using polymerase chain reaction (PCR). PCR products
were subjected to enzymatic digestion using RsaI endonuclease. Digested PCR
products were electrophoresed on 2% agarose gel. The results showed the
existence of 10 alleles: A, B, E, F, I, M, O, P, Q and V for the exon 2 of
the MHC gene, with the frequencies of 0.4756, 0.0976, 0.0183, 0.0366,
0.0549, 0.0122, 0.1098, 0.0915, 0.0854 and 0.0183, respectively. Eighteen
genotypes: AA, AB, AE, FF, AM, BO, EO, IO, OM, AP, BP, OP, PP, AQ, OQ, PQ,
QQ and AV with the frequencies of 0.317, 0.1585, 0.0121, 0.0365, 0.0121,
0.0243, 0.0243, 0.1097, 0.0121, 0.0487, 0.0121, 0.0365, 0.0365, 0.0487,
0.0121, 0.0121, 0.0487 and 0.0365, respectively were identified in the
population under study. Effective number of alleles and heterozygosity for
the examined region were 3.7231 and 0.7314, respectively. Chi-square test
showed that the examined sheep population was not in Hardy-Weinberg
equilibrium in the examined region. <br><br><font color="red"><b> This article has been corrected. Link to the correction <u><a href="http://dx.doi.org/10.2298/GENRSR1602800A">10.2298/GENRSR1602800A</a><u></b></font>
Publisher
National Library of Serbia