CANINE MALASSEZIA DERMATITIS TREATMENT PLAN IMPROVING

Abstract

The paper presents the results of canine Malassezia dermatitis treatment with propolis tincture. To confirm the CMD diagnosis test material was taken with a glue tape from the affected skin of dogs with a generalized form of CMD. The affected ear canals were sampled with sterile cotton swabs. The affected skin was washed off and immediately screened into culture media. In the department's laboratory smears were prepared and stained according to Giemsa, Gram, and alkaline methylene blue techniques. The test material was screened on Sabouraud agar supplemented with polymyxin and lactose, modified Dixon media. Cultures were incubated in a thermostat at 370C for 24 to 72 hours. The growth pattern of the selected Malassezia cultures was observed. We checked the immunodeficiency state in dogs with a discrete method of immunoglobulins detection in the blood serum using PEG-6000. To determine the level of immunoglobulins and to determine the optimal treatment plan, 4 groups of dogs were formed, 5 dogs in each, which were selected on the analogue principle. The first and the second groups included dogs with signs of external otitis. The third and the fourth had dogs with a generalized form of CMD.Microscopy of the smears demonstrated Malassezia yeasts, in the form of small nesting doll-like or ellipsoidal cells with gram-positive staining. Loose colonies of gray- white color with a velvety border were formed in Sabouraud agar medium with glucose. Smooth colonies with wavy margins were formed in Dixon agar, the colonies were gray and white in color with a slightly yellowish tint. The isolated strain had catalase activity.Dogs in the second and third groups were treated with an immunostimulant. The results of the studies showed that the best treatment plan for dogs with ear canal inflammation was for the second group, where 3 drops of Surolan ear drops in each ear canal twice a day, 2% chlorhexidine solution, and propolis tincture once a day were used. This plan proved to be more effective, as the time of treatment was reduced. For efficacy control apart from the absence of external otitis clinical signs, mycological studies were also considered.Dogs in the third group had generalized skin lesions with lichenification and hyperpigmentation of the ventral parts of the body. To treat this form of Malassezia dermatitis, we used a complex of drugs: Skingard spray, 3 times a day to the affected skin for 14 days; Apocvel 0.4 - 0.6 mg/kg twice a day to relieve itching and allergic reactions; and propolis tincture for better skin regeneration. Apocvel was used to relieve itching and allergic reactions.