Author:
Gupta Ashish,Sangwan Manohar Lal,Varshney Rajat,Kumar Aman,Dubey Tripurari
Abstract
A wide variety of environmental or contagious microorganisms implicated in mastitis, impede the economic growth of dairy sector. Identification of polymorphism in candidate gene of host’s immune system and to rule out mastitis resistant allelic form of candidate gene usually remains prime focal point of research. Bovine peptidoglycan recognition protein-1 (PGLYRP-1), exclusively present in the granules of polymorphonuclear leukocytes has direct microbicidal properties. The present study was carried out to find the association between PGLYRP-1 polymorphic alleles with mastitis. Milk samples for somatic cell count and blood samples for PCR-RFLP analysis of PGLYRP-1 gene were collected from 20 mastitis negative and 20 mastitis positive Murrah buffaloes. There was significant difference in somatic cell count of mastitis and mastitis free animals. All amplified PCR products of ~862 bp size of partial region of PGLYRP-1 gene were subjected to each restriction enzyme (HincII or TaqαΙ or ApaI). Polymorphism in the partial region of PGLYRP-1 gene had not been established using PCR-RFLP as uniformity in pattern of digested fragments was seen. Target sequence PGLYRP-1 gene of Murrah buffalo was cloned and sequenced. BLAST analysis revealed sequence identity of PGLYRP-1 of Murrah buffalo with Bos taurus (JN085441.1) sequence at NCBI was 96%, 96% with Bos indicus (JN085440.1) and 96% with Bos indicus X Bos taurus (EU746454.1). In phylogenetic tree, the target sequence of PGLYRP-1 gene of Bubalus bubalis are found more closely related to Bos taurus than to Bos indicus.
Publisher
Office of the Library, Kasetsart University
Reference24 articles.
1. Agrawal, A., R. Varshney, P. Kirthika, R. Gupta, S. Sulabh, R. Varshney, S. Chakravarti and S. Thankappan. 2019. Global scenario of paratuberculosis: a threat to livestock sector. Biol. Rhythm Res., 52(6): 957-972 DOI: 10.1080/09291016.2019.1610858
2. Alluwaimi, A.M., C.M. Leutenegger, T.B. Farver, P.V. Rossitto, W.L. Smith and J.S. Cullor. 2003. The cytokine markers in Staphylococcus aureus mastitis of bovine mammary gland. J. Vet. Med., 50(3): 105-111. DOI: 10.1046/j.1439-0450.2003.00628.x
3. Bhattacharya, S.S., A.F. Wright, J.F. Clayton, W.H. Price, C.I. Phillips, C.M.E. Mckeown, M. Jay, A.C. Bird, P.L. Pearson, E.M. Southern and H.J. Evans. 1984. Close genetic linkage between X-linked retinitis pigmentosa and a restriction fragment length polymorphism identified by recombinant DNA probe L1.28. Nature, 309(5965): 253-255. DOI: 10.1038/309253a0
4. Bytyqi, H., U. Zaugg, K. Sherifi, A. Hamidi, M. Gjonbalaj, S. Muji and H. Mehmeti. 2010. Influence of management and physiological factors on somatic cell count in raw milk in Kosova. Vet. Arhiv., 80(2): 173-183. Available on: https://hrcak.srce.hr/file/85438
5. Detilleux, J.C., K.J. Koehler, A.E. Freeman, M.E. Kehrli and D.H. Kelley. 1994. Immunological parameters of periparturient Holstein cattle: Genetic variation. International Journal of Dairy Science, 77(9): 2640-2650. DOI: 10.3168/jds.S0022-0302(94)77205-2