Muscle hardness measurement by using ultrasound elastography: a feasibility study

Abstract

Background Muscle hardening, including delayed onset muscle soreness, can be found after vigorous exercise. Some techniques for measuring muscle hardness have been proposed. Freehand ultrasound (US) elastography has been developed and applied to breast imaging. Purpose To evaluate the feasibility of US elastography for investigating changes in muscle hardness after eccentric exercise of the elbow flexor muscles. Material and Methods Six healthy male volunteers performed eccentric contractions of the elbow flexor muscles of their non-dominant arms. US elastography was performed by using 14-6 MHz linear array transducer combined with a stabilizer and a gel pack of reference agent. Color-coded elasticity images and the relative strain ratio of the biceps brachii muscle to the reference were obtained. To verify the US elastography measurements, a commercially available durometer was also employed. Both measurements were performed before, immediately after, and 1– 4 days after exercise. The mean scores of strain ratios of the US elastography and durometer measurements were examined using repeated-measures ANOVA. Results US elastography demonstrated significant muscle hardening and recovery after exercise ( P < 0.01). Muscle hardness increased up immediately after the exercise and continued to increase, peaking on day 2 post-exercise, and then decreased until day 4. The durometer indicated similar changes to US elastography. The control arms did not demonstrate any significant change. Conclusion US elastography is feasible to measure muscle hardness and to produce a two-dimensional hardness map of the muscle.