CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis
Author:
Bertrand Julien Y.1, Kim Albert D.1, Teng Shutian1, Traver David1
Affiliation:
1. Section of Cell and Developmental Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093-0380,USA.
Abstract
Development of the vertebrate blood lineages is complex, with multiple waves of hematopoietic precursors arising in different embryonic locations. Monopotent, or primitive, precursors first give rise to embryonic macrophages or erythrocytes. Multipotent, or definitive, precursors are subsequently generated to produce the adult hematopoietic lineages. In both the zebrafish and the mouse, the first definitive precursors are committed erythromyeloid progenitors (EMPs) that lack lymphoid differentiation potential. We have previously shown that zebrafish EMPs arise in the posterior blood island independently from hematopoietic stem cells (HSCs). In this report, we demonstrate that a fourth wave of hematopoietic precursors arises slightly later in the zebrafish aorta/gonad/mesonephros (AGM) equivalent. We have identified and prospectively isolated these cells by CD41(itga2b) and cmyb expression. Unlike EMPs, CD41+ AGM cells colonize the thymus to generate rag2+ T lymphocyte precursors. Timelapse imaging and lineage tracing analyses demonstrate that AGM-derived precursors use a previously undescribed migration pathway along the pronephric tubules to initiate adult hematopoiesis in the developing kidney, the teleostean equivalent of mammalian bone marrow. Finally, we have analyzed the gene expression profiles of EMPs and AGM precursors to better understand the molecular cues that pattern the first definitive hematopoietic cells in the embryo. Together, these studies suggest that expression of CD41 and cmyb marks nascent HSCs in the zebrafish AGM, and provide the means to further dissect HSC generation and function in the early vertebrate embryo.
Publisher
The Company of Biologists
Subject
Developmental Biology,Molecular Biology
Reference70 articles.
1. Al-Adhami, M. A. and Kunz, Y. W. (1977). Ontogenesis of haematopoietic sites in Brachydanio rerio. Dev. Growth Differ.19,171-179. 2. Amsen, D., Antov, A., Jankovic, D., Sher, A., Radtke, F.,Souabni, A., Busslinger, M., McCright, B., Gridley, T. and Flavell, R. A.(2007). Direct regulation of Gata3 expression determines the T helper differentiation potential of Notch. Immunity27, 89-99. 3. Bertrand, J. Y., Giroux, S., Cumano, A. and Godin, I.(2005a). Hematopoietic stem cell development during mouse embryogenesis. Methods Mol. Med.105,273-288. 4. Bertrand, J. Y., Jalil, A., Klaine, M., Jung, S., Cumano, A. and Godin, I. (2005b). Three pathways to mature macrophages in the early mouse yolk sac. Blood106,3004-3011. 5. Bertrand, J. Y., Giroux, S., Golub, R., Klaine, M., Jalil, A.,Boucontet, L., Godin, I. and Cumano, A. (2005c). Characterization of purified intraembryonic hematopoietic stem cells as a tool to define their site of origin. Proc. Natl. Acad. Sci. USA102,134-139.
Cited by
196 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|