Liprin-α1, ERC1 and LL5 identify a polarized, dynamic compartment implicated in cell migration

Abstract

Cell migration during development and metastatic invasion requires the coordination of actin and adhesion dynamics to promote the protrusive activity at the cell front. The knowledge of the molecular mechanisms required to achieve such coordination is fragmentary. Here we identify a new functional complex that drives cell motility. The adaptor proteins ERC1a and LL5 are required with liprin-α1 for effective migration and tumor cell invasion, and do so by stabilizing the protrusive activity at the cell front. Depletion of either protein negatively affects invasion, migration on extracellular matrix, lamellipodial persistence, as well as the internalization of active integrin β1 receptors needed for adhesion turnover at the cell front. Liprin-α1, ERC1a and LL5 also define new highly polarized and dynamic cytoplasmic structures uniquely localized near the protruding cell edge. Our results indicate that the functional complex and the associated structures described here represent an important mechanism to drive tumor cell migration.