Changes in cell shape are correlated with metastatic potential in murine and human osteosarcomas

Author:

Lyons Samanthe M.12,Alizadeh Elaheh3,Mannheimer Joshua1,Schuamberg Katherine1,Castle Jordan4,Schroder Bryce1,Turk Philip5,Thamm Douglas2,Prasad Ashok13

Affiliation:

1. School of Biomedical Engineering, Colorado State University, Fort Collins, CO 80523, USA

2. College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA

3. Department of Chemical and Biological Engineering, Colorado State University, Fort Collins, CO 80523, USA

4. Department of Biology, Colorado State University, Fort Collins, CO 80523, USA

5. Department of Statistics, Colorado State University, Fort Collins, CO 80523, USA

Abstract

ABSTRACT Metastatic cancer cells for many cancers are known to have altered cytoskeletal properties, in particular to be more deformable and contractile. Consequently, shape characteristics of more metastatic cancer cells may be expected to have diverged from those of their parental cells. To examine this hypothesis we study shape characteristics of paired osteosarcoma cell lines, each consisting of a less metastatic parental line and a more metastatic line, derived from the former by in vivo selection. Two-dimensional images of four pairs of lines were processed. Statistical analysis of morphometric characteristics shows that shape characteristics of the metastatic cell line are partly overlapping and partly diverged from the parental line. Significantly, the shape changes fall into two categories, with three paired cell lines displaying a more mesenchymal-like morphology, while the fourth displaying a change towards a more rounded morphology. A neural network algorithm could distinguish between samples of the less metastatic cells from the more metastatic cells with near perfect accuracy. Thus, subtle changes in shape carry information about the genetic changes that lead to invasiveness and metastasis of osteosarcoma cancer cells.

Funder

Directorate for Mathematical and Physical Sciences

Publisher

The Company of Biologists

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology

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