Sharing the mitotic pre-ribosomal particles between daughter cells

Author:

Sirri Valentina1,Jourdan Nathalie2,Hernandez-Verdun Danièle3,Roussel Pascal1

Affiliation:

1. Univ. Paris Diderot, Unit of Functional and Adaptive Biology, UMR 8251 CNRS, 4 rue Marie-Andrée Lagroua Weill-Hallé, F-75205 Paris, France

2. UPMC Univ. Paris 06, Institut de Biologie Paris Seine, UMR 8256 CNRS, 9 quai St Bernard, F-75252 Paris, France

3. Univ. Paris Diderot, Institut Jacques Monod, UMR 7592 CNRS, 15 rue Hélène Brion, F‑75205 Paris, France

Abstract

Ribosome biogenesis is a fundamental multistep process initiated by the synthesis of 90S pre-ribosomal particles in the nucleoli of higher eukaryotes. Even though synthesis of ribosomes stops during mitosis while nucleoli disappear, mitotic pre-ribosomal particles persist as observed in prenucleolar bodies (PNBs) during telophase. To further understand the relationship between the nucleolus and the PNBs, the presence and the fate of the mitotic pre-ribosomal particles during cell division was investigated. We demonstrate that the recently synthesized 45S precursor ribosomal RNAs (pre-rRNAs) but also the 32S and 30S pre-rRNAs are maintained during mitosis and associated with the chromosome periphery together with pre-rRNA processing factors. Maturation of the mitotic pre-ribosomal particles, as assessed by the stability of the mitotic pre-rRNAs, is transiently arrested during mitosis by a cyclin-dependent kinase (CDK)1-cyclin B-dependent mechanism and may be restored by CDK inhibitor treatments. At the M/G1 transition, the resumption of mitotic pre-rRNA processing in PNBs does not induce the disappearance of PNBs that only occurs when functional nucleoli reform. Strikingly, during their maturation process, mitotic pre-rRNAs localize in reforming nucleoli.

Funder

This study was supported in part by grants from the Centre National de la Recherche Scientifique, the UPMC Universite Paris 06 and the Universite Paris Diderot.

Publisher

The Company of Biologists

Subject

Cell Biology

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